吉林11选5开奖结果

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ELISA试剂盒有哪些操作应用领域

ELISA试剂盒有哪些操作应用领域

发布时间:2019-05-15

ELISAFAJUYOULINGMIN、TEYI、JIANDAN、KUAISU、WENDINGJIYIYUZIDONGHUACAOZUODENGTEDIAN。BUJINSHIYONGYULINCHUANGBIAOBENDEJIANCHA,ERQIEYOUYUYITIANZHINEIKEYIJIANCHAJIBAISHENZHISHANGQIANFENBIAOBEN,YINCI,YESHIHEYUXUEQINGLIUXINGBINGXUEDIAOCHA。ELISAFASHIMIANYIZHENDUANZHONGDEYIXIANGXINJISHU,XIANYICHENGGONGDIYINGYONGYUDUOZHONGBINGYUANWEISHENGWUSUOYINQIDECHUANRANBING、JISHENGCHONGBINGJIFEICHUANRANBINGDENGFANGMIANDEMIANYIZHENDUAN。YEYIYINGYONGYUDAFENZIKANGYUANHEXIAOFENZIKANGYUANDEDINGLIANGCEDING,GENJUYIJINGSHIYONGDEJIEGUO,BENFABUJINKEYIYONGLAICEDINGKANGTI,ERQIEYEKEYONGYUCEDINGTIYEZHONGDEXUNHUANKANGYUAN,SUOYIYESHIYIZHONGZAOQIZHENDUANDELIANGHAOFANGFA。YINCIELISAFAZAISHENGWUYIXUEGELINGYUDEYINGYONGFANWEIRIYIKUODA,KEGAIKUOSIGEFANGMIAN:

1、MIANYIMEIRANSEGEZHONGXIBAONEICHENGFENDEDINGWEI。

吉林11选5开奖结果2、YANJIUKANGMEIKANGTIDEHECHENG。

3、XIANXIANWEILIANGDEMIANYICHENDIANFANYING。

吉林11选5开奖结果4、DINGLIANGJIANCETIYEZHONGKANGYUANHUOKANGTICHENGFEN。

 

操作流程如下:

吉林11选5开奖结果(1)DAICEYANGPINKONGZHONGMEIKONGJIARUDAICEYANGPIN100μl,MEIZHONGYANGPINSHE3GEPINGXINGKONG;SHELIANGGEYINXINGDUIZHAOKONG,MEIKONGJIAWEICHULIZUDEXIBAOLIEJIEYE100μl;LINGSHEYIGEKONGBAIDUIZHAOKONG,JIARUCHUNXIBAOLIEJIEYE100μl。 (2)MEIBIAOBANZHI4℃,BAOBEIGUOYE。

(3)XIBAN:XIGANKONGNEIFANYINGYE,YONGXIDIYEGUOXIYIBIAN(JIANGXIDIYEZHUMANBANKONGHOU,JISHUAIQU),ZHIHOUJIANGXIDIYEZHUMANBANKONG,JINPAO1-2FENZHONG,JIANXIEYAODONG。SHUAIQUKONGNEIYETIHOUZAIXISHUIZHISHANGPAIGAN。ZHONGFUXIDI3-4CI。

吉林11选5开奖结果(4)YINXINGDUIZHAOKONGMEIKONGJIARUPBS 50μl,YANGPINKONGJIKONGBAIKONGMEIKONGJIARU1:500XISHIDETUKANGRENAIFKANGTIGONGZUOYE50μl。 

吉林11选5开奖结果(5)MEIBIAOBANZHI37℃PEIYANGXIANGDESHIHENEI,FUYU60min。 

(6)XIBAN,TONG(4)。 

(7)MEIKONGJIA1:5000XISHIDEHRP-BIAOJIDESHANYANGKANGTUKANGTIGONGZUOYE 100μl。 

吉林11选5开奖结果(8)MEIBIAOBANZHI37℃PEIYANGXIANGDESHIHENEI,FUYU60min。 

吉林11选5开奖结果(9)XIBAN,TONG(4)。 

吉林11选5开奖结果(10)MEIKONGJIATMBXIANSEYE 100μl,QINGQINGHUNYUN10s,ZHI37℃ANCHUFANYING15-20min。 

吉林11选5开奖结果(11)MEIKONGJIA100μl 2mol/L H2SO4ZHONGZHIFANYING。 

(12)FENBIECE450nm XIGUANGZHIW1HE630nmXIGUANGZHIW2,ZUIZHONGCEDEDEODZHIWEILIANGZHEZHICHA(W1-W2),YIJIANSHAOYOURONGQISHANGDEHUAHENHUOZHIYINDENGZAOCHENGDEGUANGGANRAO。

(13)SHUJUCHULI:ZAIDECHUBIAOBEN(S)HEYINXINGDUIZHAO(N)DE ODZHIHOU,JISUANS/NZHI。S/N≥2.1WEIYANGXINGPANDINGBIAOZHUN。

 

注:所用溶液配方

(1) PBS:CHENGQU0.2g KH2P04, 2.9g Na2HP04?12H20、8.0g NaCl, 0.2g KCl,JIASHUANGZHENGSHUIZHI1000ml。

(2)BAOBEIHUANCHONGYE:CHENGQU1.59g Na2CO3, 2.938 NaHCO3,JIASHUANGZHENGSHUIZHI 1000m1。

吉林11选5开奖结果(3)XIDIYE(PBST):HAN0.05% Tween-20DEPBS。

(4)XISHIYE:HAN0.1%NIUXUEQINGBAIDANBAI(BSA)DEPBS,ZHUYAOYONGYIXISHIKANGTI、BIAOZHUNPIN、YANGPIN。

吉林11选5开奖结果(5)TMBXIANSEYE:CHENGQU1.84g Na2HP04.12H20, 0.51 gNINGMENGSUAN,JIASHUANGZHENGSHUIZHI1O0ml,PEICHENGNINGMENGSUAN-LINSUANHUANCHONGRONGYE。YONG10.5 ml NINGMENGSUAN-LINSUANHUANCHONGRONGYERONGJIE1DINGTMB,ZAIJIARU35μl 3% H2O2

(6)XIBAOLIEJIEYE:1% TritonX-100,137mmol/L NaCl,20mmol/L Tris-HCl,2mmol/L EDTA,1mmol/L PMSF,pH 7.4。(QIZHONGPMSFRONGYUYIBINGCHUNZHONG,PEICHENG1O mmo/L,-20℃ ZHUCUNBEIYONG。